Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

IgG, (lambda chain specific) (TRITC)

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Referência I1904-35-1mg

Tamanho : 1mg

Marca : US Biological

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I1904-35 IgG, (lambda chain specific) (TRITC)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

E IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label:
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)

Absorption Wavelength:
550nm

Emission Wavelength:
570nm

Fluorochrome/Protein Ratio:
4.0 moles TRITC per mole of Goat IgG F(ab’)2

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free),|0.01% Sodium Azide. Labeled with TRITC.|Purity: Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation|Immunogen: Human λ (lambda chain)|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti- Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human λ (lambda chain)

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free),|0.01% Sodium Azide. Labeled with TRITC.

Purity

Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti- Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.