store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
IPTG [367-93-1]
Referência T4336-1mL
Tamanho : 1mL
Marca : TargetMol
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IPTG
Catalog No. T4336 CAS 367-93-1
Synonyms: Isopropyl β-D-thiogalactoside
IPTG (Isopropyl β-D-thiogalactoside) is a non-metabolizable galactose analog that induces expression of the LAC OPERON.
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IPTG, CAS 367-93-1
| Description | IPTG (Isopropyl β-D-thiogalactoside) is a non-metabolizable galactose analog that induces expression of the LAC OPERON. |
| In vitro | At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease. |
| Kinase Assay | For RNA interference (RNAi), A549 cells are plated at 1,500 cells per well in 96-well plates, allowed to adhere for 24 hours, and transfected with 25 nM siRNA oligonucleotide using Dharmafect 4. Transfected cells are treated with the indicated concentrations of GNE-617 (0.1, 1 , 10 , 100 , and 1000 nM) for 72 hours and viability is evaluated with CellTiter-Glo. Lysates for detection of NAPRT1 protein are collected 72 hours after transfection of 1 million A549 cells in 10 cm dishes. For NAPRT1 re-expression, RERF-LC-MS cells are transfected with pCMV6-AC.NAPRT1 and empty vector pCMV6-AC using Amaxa Nucleofector technology and selected with Geneticin |
| Synonyms | Isopropyl β-D-thiogalactoside |
| Molecular Weight | 238.3 |
| Formula | C9H18O5S |
| CAS No. | 367-93-1 |
Storage
Solubility Information
DMSO: 60 mg/mL (251.78 mM)
References and Literature