OCOA14478 | KLF5 Protein Lysate Clinisciences

Datasheets/Manuals	Printable datasheet for KLF5 Protein Lysate (OCOA14478)

Product Info
Predicted Species Reactivity	Homo sapiens
Product Format	Lyophilized
Host	HEK293T
Additional Information	Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::	Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage	2°C to 8°C
Tag	C-MYC/DDK

Gene Symbol	KLF5
Gene Full Name	Kruppel like factor 5
Alias Symbols	(intestinal Kruppel-like factor, basic transcription element binding protein 2, Basic transcription element-binding protein 2, BTE-binding protein 2, BTEB2, CKLF, colon krueppel-like factor, colon kruppel-like factor, epididymis secretory sperm binding protein, GC box binding protein 2, GC-box-binding protein 2, IKLF, intestinal-enriched krueppel-like factor, intestinal-enriched kruppel-like factor, Krueppel-like factor 5, Kruppel-like factor 5 (intestinal), transcription factor BTEB2.
NCBI Gene Id	688
Protein Name	Homo sapiens Kruppel like factor 5 (KLF5), transcript variant 1, mRNA\|Krueppel-like factor 5\|Krueppel-like factor 5 isoform 1 [Homo sapiens]
Description of Target	Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Uniprot ID	Q13887
Protein Accession #	https://www.ncbi.nlm.nih.gov/protein/NP_001721
Nucleotide Accession #	https://www.ncbi.nlm.nih.gov/nuccore/NM_001730
Molecular Weight	50.6 kDa

KLF5 Protein Lysate