Magic Red™ Cathepsin L BioAssay™ Kit

Sample Type:
Cell culture

Reagent Name:
MR-FR2

Target:
Cathepsin L

Excitation/Emission:
592nm/628nm

Method of Analysis:
Fluorescent Plate Reader, Fluorescence Microscope

Intended Use:
Quantitate and monitor intracellular cathepsin L activity over time in vitro. The Magic Red reagent in this BioAssay™ fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescent plate reader.

Test Principle:
Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions. Magic Red BioAssay™ kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This Magic Red kit detects cathepsin L

The Magic Red reagent MR-FR2 enters each cell in a non-fluorescent state. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophone will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or fluorescent plate reader. Samples may also be analyzed with Acridine Orange or Hoechst stain to detect lysosomal organelle structure or nuclear morphology respectively.

Kit Components:
25 tests:
Magic Red Reagent (MR-FR2), 1 25-test vial
Hoechst 33342 Stain, 1x1mL
Acridine Orange Stain, 1x0.5mL

100 tests:
Magic Red Reagent (MR-FR2), 1 100-test vial
Hoechst 33342 Stain, 1x1mL
Acridine Orange Stain, 1x0.5mL

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.