Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16)
Figure-1: Western blot analysis of Spectrin beta 3. Anti-Spectrin beta 3 antibody (Clone: ABM5A16) was used at 0.1 µg/ml on h Kidney and h Brain lysates.
| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2a, kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | SPTBN2 |
| Gene ID : | 6712 |
| Uniprot ID : | O15020 |
| Alternative Name : | Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Spinocerebellar ataxia 5 protein |
| Immunogen Information : | A partial length recombinant protein (a.a 1280-1450) of Spectrin beta 3 was used as the immunogen for this antibody. |
Beta-III spectrin is a protein encoded by the gene SPTBN2, spans 2390 amino acids and consists of an amino-terminal actin binding domain (ABD), a central region containing seventeen spectrin repeat domains, and a carboxy-terminal pleckstrin homology domain. It is critical for the correct development and maintenance of Purkinje cell dendritic structure. Beta-III spectrin is expressed predominantly in the brain and is enriched in cerebellar Purkinje cells. In addition, Beta-III spectrin participates in endomembrane trafficking through its interaction with the actin related protein, ARP1. The functional unit of Beta-III spectrin is considered to be a tetrameric complex composed of two Beta-spectrin subunits and two Alpha-II-spectrin subunits. Mutations in the gene encoding Beta-III spectrin give rise to spinocerebellar ataxia type 5, a neurodegenerative disease characterized by progressive thinning of the molecular layer, loss of Purkinje cells and increasing motor deficits.
WB: 0.1-0.5 µg/ml, FACS: 2-4 µg/10^6
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm |
| Tissue Specificity: | Highly expressed in brain, kidney, pancreas, and liver, and at lower levels in lung and placenta. |
| BioGrid: | 112590. 34 interactions. |
| There are currently no product reviews |