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> Mouse Human secretory component (free and bound), conjugated with Biotin Antibody

Mouse Human secretory component (free and bound), conjugated with Biotin Antibody

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Referência orb21193-0.2mg

Tamanho : 0.2mg

Marca : Biorbyt


Mouse Human secretory component (free and bound), conjugated with Biotin Antibody

SKU: orb21193
This product can be custom made

Description

Biotin-conjugated purified monoclonal mouse antibody to human secretory component, free or bound. To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Images & Validation

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Mouse Human secretory component (free and bound), conjugated with Biotin Antibody
Tested ApplicationsDOT, ELISA, ICC, IHC, WB
ReactivityHuman

Key Properties

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Antibody TypeSecondary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.NI 194-4 (A89-39)
ConjugationBiotin

Storage & Handling

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StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
Form/AppearancePurified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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