Mouse IgE Antibody : Biotin
Referência OASB00170
Tamanho : 0.5mg
Marca : Aviva Systems Biology
Mouse IgE Antibody : Biotin (OASB00170)
| Datasheets/Manuals | Printable datasheet for Mouse IgE Antibody : Biotin (OASB00170) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 23G3 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | Biotin |
| Application | FC, IHC-F, IHC-P, IHC, WB, ELISPOT |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Monoclonal anti-DNP mouse IgE |
| Concentration | 0.5 mg/mL |
| Specificity | IgE |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA or FLISA for conformance tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | FLISA: ELISA: Fluorescein conjugate R-phycoerythrin conjugate Alkaline phosphatase conjugate Horseradish peroxidase conjugate Biotin conjugate 1:200 – 1:400 <=1ug/mL 1:500-1:1,000 1:1,000-1:2,000 1:5,000-1:10,000 |
| Application Info | Identification and enumeration of IgE+ cells by flow cytometry, Identification and enumeration of IgE+ cells by immunofluorescence microscopy, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA) |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
