RayBio® G-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.
Mouse Inflammation Array G1
Mouse Inflammation Array G1
Contact Us for Bulk Request
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Fas Ligand (TNFSF6)
|
| Fractalkine (CX3CL1)
| GCSF
| GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
|
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-6
|
| IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
| IL-17A
| I-TAC (CXCL11)
| KC (CXCL1)
| Leptin
| LIX
|
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
| MIP-1 gamma
| RANTES (CCL5)
| SDF-1 alpha (CXCL12 alpha)
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
|
| TIMP-1
| TIMP-2
| TNF alpha
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Inflammation Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAM-INF-G1-4.gal)
GAL (GAL-AAM-INF-G1-8.gal)
Excel (AAM-INF-G1_Analysis_Tool.xls)
citations
Assessing the Adverse Impacts of PM2.5 on Olfactory System Using an Air-liquid Interface Culture Model of Primary Olfactory Epithelial Cells
- Species:
- Mouse
- Sample Type:
- olfactory epithelial cell(OEC)
- Year:
- 2023
- Journal Name:
- Chemical Research in Chinese Universities
Aryl hydrocarbon receptor dependent anti-inflammation and neuroprotective effects of tryptophan metabolites on retinal ischemia/reperfusion injury
- Species:
- Mouse
- Sample Type:
- BV2 microglia cell
- Year:
- 2023
- Journal Name:
- Cell Death & Disease
Interference with LTβR signaling by tick saliva facilitates transmission of Lyme disease spirochetes
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Apolipoprotein A-IV reduced metabolic inflammation in white adipose tissue by inhibiting IKK and JNK signaling in adipocytes
- Species:
- Mouse
- Sample Type:
- RAW264.7 macrophages
- Year:
- 2022
- Journal Name:
- Molecular and Cellular Endocrinology
Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke
- Species:
- Mouse
- Sample Type:
- brain tissue
- Year:
- 2022
- Journal Name:
- Brain Research Bulletin
Are Fried Foods Unhealthy The Dietary Peroxidized Fatty Acid, 13-HPODE, Induces Intestinal Inflammation In Vitro and In Vivo
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2020
- Journal Name:
- Antioxidants
Microglial NMDA receptors drive proinflammatory responses via PARP1/TRMP2 signaling
- Species:
- Mouse
- Sample Type:
- Conditioned media (cell culture media)
- Year:
- 2020
Characteristics of Changes in Inflammatory Cytokines as a Function of Hepatic Ischemia-Reperfusion Injury Stage in Mice
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Liver)
- Year:
- 2019
- Journal Name:
- Inflammation
Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury
- Species:
- Mouse
- Sample Type:
- BAL Fluid
- Year:
- 2017
- Journal Name:
- Cell Death and Disease
Timing underpins the benefits associated with injectable collagen biomaterial therapy for the treatment of myocardial infarction
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Heart infarct Tissue lyates)
- Year:
- 2015
- Journal Name:
- Biomaterials
- PubMed ID:
- 25468370