Mouse Ly-6A/E Antibody : Biotin
Referência OASB00553
Tamanho : 0.5mg
Marca : Aviva Systems Biology
Mouse Ly-6A/E Antibody : Biotin (OASB00553)
| Datasheets/Manuals | Printable datasheet for Mouse Ly-6A/E Antibody : Biotin (OASB00553) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | D7 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | Biotin |
| Application | FC, IHC-F, IHC-P, IP, WB |
| Additional Information | Description: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L |
| Concentration | 0.5 mg/mL |
| Specificity | Ly-6A/E |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative flow data are included in this productinsert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate R-phycoerythrin-Cyanine 5.5 conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1, 4-7, Immunoprecipitation 1, Western blotting 1, 2, Immunohistochemistry (frozen sections) 14, In vitro lymphocyte activation 9-12 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: ≤0.5 Ug/106 cells BALB/c spleen cells were activated by incubation for 48 hours with concanavalin-A. After harvesting, the cells were stained with rat anti-mouse Ly6A/E-FITC, and activated cells were gated and analyzed on a FACScan f |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates are suppliedas 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Ortega, G., P.E. Korty, E.M. Shevach, and T.R. Malek. 1986. J. Immunol. 137:3240- 3246 2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197- 207 3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195- 224 4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653- 1663 5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412- 418 6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915- 1921 7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98- 107 8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929- 1936 9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197- 2204 10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709- 722 11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407- 1414 12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394- 2406 13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115- 120 14. van Bragt,M.P.A., N. Ciliberti, W.L. Stanford, D.G. de Rooij, and A.M.M. van Pelt. 2005. Biol Reprod 73:634. |
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| Gene Symbol | LY6A, LY6E |
| Gene Full Name | Lymphocyte antigen 6A-2/6E-1 |
| Alias Symbols | T, Sc, Sca, TAP, Sca1, Ly-6A, Sca-1, Ly-6E., Ly-6A.2, Ly-6A/E, Ly-6E.1 |
| NCBI Gene Id | 110454; 17069 |
| Protein Name | lymphocyte antigen 6A-2/6E-1 |
| Uniprot ID | P05533, Q64253 |
| Protein Accession # | NP_001258345.1 |
| Nucleotide Accession # | NM_001271416.1 |
