Notch1 antibody
Referência orb150328-100ug
Tamanho : 100ug
Marca : Biorbyt
Notch1 antibody
Catalog Number: orb150328
Catalog Number | orb150328 |
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Category | Antibodies |
Description | Mouse monoclonal to Notch1 (ATTO-488). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | S253-32 |
Tested applications | ICC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000) |
Conjugation | ATTO-488 |
MW | 270kDa |
Target | Notch1 |
Entrez | 18128 |
UniProt ID | Q01705 |
NCBI | NP_032740.3 |
Storage | Conjugated antibodies should be stored at 4°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Alternative names | Neurogenic locus notch homolog protein 1 antibody, |
Note | For research use only |
Application notes | 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |