PerfectStart II Probe qPCR SuperMix UDG

Referência AQ712-01

Tamanho : 1ml

Marca : TransGen Biotech


5×1 ml
15×1 ml
Manual MSDS
CoA
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Product Details

PerfectStartTM II Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and

template. It contains PerfectStartTM Taq DNA Polymerase (PerfectStartTM Taq DNA polymerase is a hot-start Taq DNA polymerase

containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and

preventing non-specific amplification at low temperature), specially optimized dual-cation buffer, dNTPs, PCR enhancer and stabilizer.

qPCR SuperMix is provided at 2×concentration and can be used at 1×concentration by adding template, primer, probe, passive

reference dye (optional) and Nuclease-free Water. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA,

which can prevent cross contamination.


Highlights

PerfectStart™ Taq DNA Polymerase, hot start with three antibodies blocking technique, improves sensitivity, enhances specificity and generates more accurate data.

• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity.

• dUTP/UDG used to avoid cross contamination.

• Passive reference dyes are provided for different qPCR instruments.


Storage

at -18℃ or below for two years


Shipping

Dry ice (-70°C)

Product Composition

Experimental Data

Comparison to other brands

Using products from TransGen, Company V, and Company T, amplify different concentrations of porcine reproductive and respiratory syndrome virus (PRRSV) cDNA (10 pg, 0.1 pg, 0.01 pg) and bovine viral diarrhea virus (BVDV) cDNA (100 pg, 1 pg, 0.1 pg) as templates, conduct qPCR detection, and analyze the amplification effect and sensitivity.


FAQ
  • qPCR FAQs
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