PRC1 Antibody (HRP)
Referência orb344729-100ug
Tamanho : 100ug
Marca : Biorbyt
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, WB: 1:1,000 - 1:5,000 |
| Reactivity | Yeast |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Carboxypeptidase Y [Baker’s Yeast] |
| Target | PRC1 |
| Purity | Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C
−
PRC1 Rabbit Polyclonal Antibody (HRP) [orb468003]
IHC-Fr, IHC-P
Canine, Equine, Human
Mouse, Rat
Rabbit
Polyclonal
HRP
100 μl
Western Blot of Peroxidase conjugated Rabbit anti-Carboxypeptidase Y Antibody. Lane 1: Carboxypeptidase Y. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit Carboxypeptidase Y antibody at 1:1000 for 60 min at RT Block: orb348637 for 30 min at RT. Predicted/Observed size: 53 kDa for Carboxypeptidase Y. Other band(s): none.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_014026.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PRC1 Antibody (HRP) (orb344729)
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