RCC1 (13-21) Mouse Monoclonal Antibody [Clone ID: 3D11]
Specifications
Product Data | |
Clone Name | 3D11 |
Recommended Dilution | Western blot: 1 µg/ml for a chemiluminescence detection system. Immunocytochemistry: 5µg/ml. Immunoprecipitation: 2 μg/200 μl of cell extract from 5 x 106 cells. |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Full-length human RCC1 |
Specificity | This antibody detects N-terminal domain (13-21 aa) of RCC1. It does not inhibit GEF activity of RCC1. |
Formulation | PBS (pH 7.2) / 1% sucrose. Contains no preservative. State: Azide Free State: Lyophilized Ig fraction |
Reconstitution Method | Restore with 100 µl distilled water. |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Prior to reconstitution store at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Gene Name | regulator of chromosome condensation 1 |
Database Link | |
Background | The human RCC1 (Regulator of Chromosome Condensation) gene has been isolated as a gene that rescues tsBN2 mutation. The rcc1 - phenotype is pleiotropic and includes G 1 arrest, defects in mRNA splicing and mRNA transport. RCC1 is an abundant, highly conserved, chromatin-associat ed protein of 45 kDa which possesses an N-terminal region of 40 amino acid residues and an internal repeated doma in in which about 60 amino acid residues are repeated seven times (RCC1 repeat). RCC1 is the principle mammalian guanine nucleotide exchange factor (GEF) for the nuclear G protein Ran, which GTPase activity is enhanced by GTPase-activating protein RanGAP located in the cytoplasm. RCC1 plays an important role in RNA transcription and processing, DNA replication, nuclear pore transport function and cell cycle regulation on Ran pathway. |
Synonyms | RCC1-I |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunocytochemistry Immunoprecipitation |
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