Orb1222127-100mg | TCEP HCl [51805-45-9] Clinisciences

TCEP HCl

SKU: orb1222127

Description

Tris(2-carboxyethyl)phosphine hydrochloride may be employed as a catalyst for the reduction of sulfoxides, sulfonyl chlorides, N-oxides, and azides. It may be used as a reducing agent for the reduction of various alkyl disulfides such as trans-4, 5-dihydroxy-1, 2-dithiane.

Images & Validation

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Key Properties

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CAS Number	51805-45-9
MW	286.65
Purity	>98% (HPLC)
Formula	C₉H₁₆ClO₆P
SMILES	Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
Target	Caspase
Solubility	In Vitro: DMSO : 100 mg/mL (348.86 mM)

Bioactivity

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In Vitro	TCEP hydrochloride has been introduced which o ers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions. The human lactoferrin (hLF) peptide is dissolved in phosphate buffer to a concentration of 0.1 mm. Reduction of the disulfide bonds is obtained by adding a 30-fold molar excess of TCEP hydrochloride with subsequent incubation for 2 h at 37 °C.

In Vitro

TCEP hydrochloride has been introduced which o ers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions. The human lactoferrin (hLF) peptide is dissolved in phosphate buffer to a concentration of 0.1 mm. Reduction of the disulfide bonds is obtained by adding a 30-fold molar excess of TCEP hydrochloride with subsequent incubation for 2 h at 37 °C.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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TCEP HCl [orb1300086]
Purity:
>99%
CAS Number:
51805-45-9
MW:
286.7
Formula:
C₉H₁₅O₆P HCl
Sizes Available: 1 g, 5 g

Quick Database Links

Gene Symbol

Caspase

TCEP HCl [51805-45-9]

Referência orb1222127-100mg

Marca : Biorbyt