Tris Buffered Saline with Tween 20: 1 L of 20X
Referência sc-281695
Tamanho : 1L
Marca : Santa Cruz Biotechnology
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Tris Buffered Saline with Tween 20: 1 L of 20X
Noms alternatifs:
20X TBST
Application:
Tris Buffered Saline with Tween 20: 1 L of 20X is a convenient prepared buffer for use in various applications
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
Tris Buffered Saline with Tween 20 at a concentration of 20X (TBST 20X) is a highly concentrated buffer solution commonly employed in biochemical and molecular biology research. The solution contains Tris (tris(hydroxymethyl)aminomethane), saline (sodium chloride), and Tween 20, a non-ionic surfactant. This composition is designed to provide a stable buffered environment with a pH typically maintained around 7.4 to 7.6, mirroring physiological conditions which are crucial for maintaining the structural integrity and functionality of biological molecules. The primary utility of TBST 20X lies in its role in immunological assays, such as Western blotting, enzyme-linked immunosorbent assays (ELISA), and immunofluorescence. The Tween 20 component is particularly important as it serves as a mild detergent. It disrupts weak hydrophobic interactions, helping to solubilize membrane proteins and reduce nonspecific binding. This action is critical for washing steps in these assays, effectively removing unbound antibodies while preserving the bound antibodies, thus ensuring clarity and specificity in signal detection. Moreover, the high concentration (20X) format of TBST allows researchers to dilute the solution to their specific needs, providing flexibility and ensuring consistent preparation of working solutions. This high concentration also aids in reducing storage space and shipping costs, while the presence of Tween 20 enhances membrane permeability and improves the quality of antigen-antibody interactions in the research applications.
2. Doran, TM, et al. Bioorg Med Chem Lett. 2016. 25(21): 4910-4917. PMID: 26067174
3. Kemper, C. et al. Bio Protoc. 2014. 4(16):e1205. PMID: 29094056
References:
1. Thomson, BR, et al. Methods Mol Biol. 2019. 1846: 153-160. PMID: 302427582. Doran, TM, et al. Bioorg Med Chem Lett. 2016. 25(21): 4910-4917. PMID: 26067174
3. Kemper, C. et al. Bio Protoc. 2014. 4(16):e1205. PMID: 29094056
Utilisation :
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 20 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
État Physique :
Liquid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
STOCKAGE :
Store at 4° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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SAMPLE Certificate of Analysis (COA)
Tris Buffered Saline with Tween 20: 1 L of 20X SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-281695
Lot Number:SAMPLE
Product Name:Tris Buffered Saline with Tween 20: 1 L of 20X
Test | Specification | Results |
---|---|---|
Appearance | Slightly yellow liquid, foams when shaken | |
pH | 7.276 | |
Sterility | Filtered with 0.2 µm filter |
TEST CONDITIONS
Exp. Date: 9/26/2027
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L | sc-281695 | 1 L | .00 |