Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Uridine [58-96-8]

Imprimir

Referência NB-64-11477-500mg

Tamanho : 500mg

Marca : Neo Biotech

Solicitar mais informações

Remove All

Uridine

(Synonyms: β-Uridine, Uridin, NSC 20256) Copy Product Info

Synonyms: β-Uridine, Uridin, NSC 20256

Catalog No. T2221 Copy Product Info

Purity: 99.99%

Uridine (Uridin) is a nucleoside compound, one of the four bases that make up RNA, that replaces thymine in DNA during transcription and pairs with adenine.

Cas No. 58-96-8

For research use only—not for human use. No sales to individuals. Use as intended only.

Select Batch

Purity:99.99%

Color:White

Product Introduction

Bioactivity

Description	Uridine (Uridin) is a nucleoside compound, one of the four bases that make up RNA, that replaces thymine in DNA during transcription and pairs with adenine.
Targets & IC50	RAW 264.7 cells:> 10 μM (EC50), P2Y14 receptor:7.28 (pEC₅₀), P2Y6:300 nM, H9 cells:10 μg/mL
In vitro	METHODS: HCC cells HEPG2, HLF and 97H were treated with Uridine (0-600 µM) for 48 h. Cell viability was measured by CCK8 assay. RESULTS: The proliferative capacity of the three HCC cells was significantly inhibited in a dose-dependent manner. [1] METHODS: Differentiated PC-12 cells were treated with Uridine (50 µM) for 4 days, and the expression levels of target proteins were measured by Western Blot. RESULTS: Uridine treatment enhanced neurofilament expression in differentiated PC12 cells, and NF-M and NF-70 expression were significantly increased after Uridine treatment. [2]
In vivo	METHODS: To detect anti-tumor activity in vivo, Uridine (100-300 mg/kg) was injected locally into BALB/c nude mice bearing 97H xenografts once daily for sixteen days. RESULTS: Both doses of Uridine significantly reduced the tumor size of subcutaneous tumors in HCC nude mice in a dose-dependent manner. [1]
Synonyms	β-Uridine, Uridin, NSC 20256
Kinase Assay	Microtubule depolymerizing activity: The effects of 2-Methoxyestradiol on cellular microtubule depolymerization are determined by indirect immunofluorescence techniques in rat aortic smooth muscle A-10 cells. Microtubules are visualized using a β-tubulin antibody. Three viewers determines the percent microtubule loss for each treatment concentration. The data are averaged and plotted as percent microtubule loss versus drug concentration and the EC50s for microtubule depolymerization calculated from the log dose–response curves.

Chemical Properties

Molecular Weight	244.20
Formula	C₉H₁₂N₂O₆
Cas No.	58-96-8
Smiles	O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C(=O)NC(=O)C=C2
Relative Density.	1.4221 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from direct sunlight,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 240.00 mg/mL (982.80 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: 45.00 mg/mL (184.28 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5.00 mg/mL (20.48 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0950 mL	20.4750 mL	40.9500 mL	204.7502 mL
5 mM	0.8190 mL	4.0950 mL	8.1900 mL	40.9500 mL
10 mM	0.4095 mL	2.0475 mL	4.0950 mL	20.4750 mL
20 mM	0.2048 mL	1.0238 mL	2.0475 mL	10.2375 mL
50 mM	0.0819 mL	0.4095 mL	0.8190 mL	4.0950 mL
100 mM	0.0410 mL	0.2048 mL	0.4095 mL	2.0475 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

NSC-20256NSC20256LSm6EndogenousMetaboliteEndogenous Metaboliteb-Uridinebeta-Uridine

Related Tags: Uridine chemical structure | Uridine in vivo | Uridine in vitro | Uridine formula | Uridine molecular weight