VEGFB ELISA Kit (Bovine)
Referência OKEH03755
Tamanho : 96Wells
Marca : Aviva Systems Biology
VEGFB ELISA Kit (Bovine) (OKEH03755)
| Datasheets/Manuals | Printable datasheet for VEGFB ELISA Kit (Bovine) (OKEH03755) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology VEGFB ELISA Kit (Bovine) (OKEH03755) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VEGFB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VEGFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VEGFB captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 13.1 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Vascular endothelial growth factor B | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | VEGFB |
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| Gene Full Name | vascular endothelial growth factor B |
| Alias Symbols | vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF. |
| NCBI Gene Id | 282121 |
| Protein Name | Vascular endothelial growth factor B |
| Description of Target | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis (By similarity). |
| Uniprot ID | Q9XS49 |
| Protein Accession # | NP_776912.1 |
| Nucleotide Accession # | NM_174487.2 |
| Protein Size (# AA) | 207 |
| Molecular Weight | 22 kDa |
