Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> WC4 (SWC7) (PE)

WC4 (SWC7) (PE)

Imprimir Imprimir
Please log in

Referência W0477-01-PE-100ul

Tamanho : 100ul

Marca : US Biological


W0477-01-PE WC4 (SWC7) (PE)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Bo Po Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Mouse anti-bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface
antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howard et al. 1993). It is suggested that WC4 like SWC7 in pigs is the bovine orthologue of human CD19 (Naessens and Howard 1991, Ikebuchi et al. 2013).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CC55|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Bovine WC4.|Specificity: Recognizes the bovine WC4 at ~90kD. Species Crossreactivity: porcine, sheep. Note: Antibody reactivity and working conditions may vary between species.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Bovine WC4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the bovine WC4 at ~90kD. Species Crossreactivity: porcine, sheep. Note: Antibody reactivity and working conditions may vary between species.
References
1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27. 2. Denham, S. et al. (1994) Monoclonal antibodies recognizing differentiation antigens on porcine B cells. Vet. Immunol. Immunopathol. 43: 259-267. 3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol. Today 8:365-368. 4. Boersma. et al. (2001) Summary of workshop findings for porcine B-cell markers. Vet. Immunol. Immunopathol. 80: 63-78.