Proteinase K (25X)
Référence GTX00761-2ml
Conditionnement : 2ml
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Application | IHC-P |
|---|---|
| Package | 2 ml |
Datasheet File View product citations for antibody GTX00761 on CiteAb
PRODUCT
Summary
Proteinase K is specifically formulated to achieve proteolytic digestion of formalin fixed, paraffin embedded tissue sections prior to performing in situ hybridization procedures. Proteolytic digestion of formalin fixed tissues improves the accessibility of target DNA to biotinylated probes, which results in better in situ staining.
APPLICATION
Application Note
Preparation of working solutions :
1. Place 100μl Proteinase K solution in a glass tube, and 2.4ml distilled water and mix.
Protocol Recommendations :
1. Optimal digestion conditions will vary depending on fixation conditions and should be optimized by the end user.
2. Incubation of tissue sections for 10 - 30 minutes at 37ºC is sufficient for in situ.
1. Place 100μl Proteinase K solution in a glass tube, and 2.4ml distilled water and mix.
Protocol Recommendations :
1. Optimal digestion conditions will vary depending on fixation conditions and should be optimized by the end user.
2. Incubation of tissue sections for 10 - 30 minutes at 37ºC is sufficient for in situ.
PROPERTIES
Form
Liquid
Storage
Store at 2-8ºC. Proteinase K is stable for 6 months. For long term storage, aliqout Proteinase K into smaller sizes and store at -20ºC. Do not use after expiration date printed on label.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.