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> Chapsyn-110 Antibody

Chapsyn-110 Antibody

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Référence ASM10201-STR-100

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  • ICC/IF - Chapsyn-110 Antibody ASM10201
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 (ASM10201). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody (ASM10201) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Chapsyn-110 Antibody (D) Composite.
  • WB - Chapsyn-110 Antibody ASM10201
    Western Blot analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 (ASM10201). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody (ASM10201) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF, AM
Primary Accession Q63622
Other Accession NP_071618.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Chapsyn-110 Monoclonal IgG1
Target/Specificity Detects ~110kDa. No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells. Weak human detection.
Other Names PSD93 Antibody, Chapsyn-110 Antibody, MGC131811 Antibody, PSD-93 Antibody, Discs Large Homolog 2 Antibody, DLG2 Antibody, Postsynaptic density protein PSD-93 Antibody, DLGH2 Antibody, Channel-associated protein of synapse-110 Antibody
Clone Names S18-30
Immunogen Fusion protein amino acids 1-852 of rat Chapsyn-110
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-325 was sufficient for detection of Chapsyn -110 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane | Cell Projection | Axon