TET recombinant proteins are research-grade proteins derived mainly from TET1 and TET2, essential enzymes involved in DNA demethylation and epigenetic regulation. Produced as catalytic fragments or tagged recombinant proteins, they provide reliable tools for investigating enzyme activity, DNA methylation dynamics, and gene regulation in normal development and disease.

Characteristics

TET proteins are Fe(II)- and α-ketoglutarate-dependent dioxygenases that catalyze the oxidation of 5-methylcytosine. Recombinant proteins are commonly expressed in E. coli or baculovirus/Sf9 systems and frequently include His, FLAG, or GST tags to facilitate purification, detection, and biochemical analyses.

Applications

• Enzyme assays: Measure TET catalytic activity and DNA demethylation efficiency.
• Drug discovery: Screen inhibitors and evaluate selectivity toward epigenetic enzymes.
• Epigenetic research: Investigate pluripotency, differentiation, cancer biology, and protein interactions.