General Info
| Host: | Rabbit |
| Applications: | WB/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-NQO1 (1-274) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | NQO1 |
| Gene ID: | 1728 |
| Uniprot ID: | NQO1_HUMAN |
| Immunogen Region: | 1-274 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1). |
| Immunogen Sequence: | MVGRRALIVLAHSERTSFNY AMKEAAAAALKKKGWEVVES DLYAMNFNPIISRKDITGKL KDPANFQYPAESVLAYKEGH LSPDIVAEQKKLEAADLVIF QFPLQWFGVPAILKGWFERV FIGEFAYTYAAMYDKGPFRS KKAVLSITTGGSGSMYSLQG IHGDMNVILWPIQSGILHFC GFQVLEPQLTYSIGHTPADA RIQILEGWKKRLENIWDETP LYFAPSSLFDLNFQAGFLM |
Description
| Function | Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion. Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential. Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome. |
| Protein Name | Nad(Ph Dehydrogenase Quinone 1AzoreductaseDt-DiaphoraseDtdMenadione ReductaseNad(Ph -Quinone Oxidoreductase 1Phylloquinone ReductaseQuinone Reductase 1Qr1 |
| Database Links | Reactome: R-HSA-350562Reactome: R-HSA-9818027 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Nad(Ph Dehydrogenase Quinone 1 antibodyAnti-Azoreductase antibodyAnti-Dt-Diaphorase antibodyAnti-Dtd antibodyAnti-Menadione Reductase antibodyAnti-Nad(Ph -Quinone Oxidoreductase 1 antibodyAnti-Phylloquinone Reductase antibodyAnti-Quinone Reductase 1 antibodyAnti-Qr1 antibodyAnti-NQO1 antibodyAnti-DIA4 antibodyAnti-NMOR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance