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> Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

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Cat# 230-30162-100

Size : 100ug

Marca : RayBiotech


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Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)This protein is used as the standard in the ELISA kit (catalog number: ELV-COVID19S1). Different diluents for different sample types.SARS-CoV-2 S1 Subunit Protein was used as the coating antigen in ELISA to measure plasma anti-RBD IgG levels. Antibody-sufficient mice showed robust responses following infection. Figure adapted from Fumagalli et al., Nature Immunology, 2024.RayBiotech's SARS-CoV-2 S1 Subunit Protein was used to stimulate human senescent endothelial cells. As shown in the heat map, S1 treatment enhanced secretion of multiple proinflammatory SASP factors. Figure adapted from Camell et al., Science, 2021.Figure adapted from Fumagalli et al., EMBO Molecular Medicine, 2023.Figure adapted from Nydegger et al., Viruses, 2023.Figure adapted from McCafferty et al., Molecular Therapy, 2022.

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells.
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Product Description

Specifications

Size100 µg, 500 µg, 1000 µg
SpeciesSARS-CoV-2
Accession Number
QHD43416
Protein Name / Synonyms
Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Expressed Region
Arg319-Phe541
Expression SystemHEK293 cells
TagC-terminal his-tag
Purity
>95 %
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Research AreaAntibody Drug Development, Receptor-Ligand Binding, Infectious Disease, COVID-19
Shipping TypeBlue ice
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Binding Function
Binding Function
Product binding ability was measured by ELISA. The immobilized recombinant human ACE2 protein (Catalog #230-30165, coated at 0.5 µg/ml, 100 µl/well) was incubated with the serial diluted SARS CoV-2 S1 RBD wild type protein (Catalog #230-30162). The bound protein was detected by mouse anti-S1 RBD monoclonal antibody using ELISA. The calculated EC50 is 32.77 ng/ml.
Activity
Sandwich ELISA: this protein functions as a standard in conjunction with 130-10815 (capture antibody) and 130-10814 (detection antibody).
Standard curve
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Introduction

SARS-CoV-2, the virus responsible for COVID-19, uses its spike protein to enter host cells, with the receptor-binding domain (RBD) playing a central role in this process. The RBD within the S1 subunit specifically binds to the angiotensin-converting enzyme 2 (ACE2) receptor on human cells, facilitating viral entry and subsequent infection. This domain is a key target for neutralizing antibodies and vaccine design.

Reference: https://link.springer.com/chapter/10.1007/978-3-031-46545-1_13

Typical Data

RayBiotech's SARS-CoV-2 S1 Subunit Protein (RBD) (Cat# 230-30162) was used as the coating antigen in ELISA to measure plasma anti-RBD IgG levels. Antibody-sufficient mice showed robust responses following infection. Figure adapted from Fumagalli et al., Nature Immunology, 2024.

RayBiotech's SARS-CoV-2 S1 Subunit Protein (RBD) (Cat# 230-30162) was used to stimulate human senescent endothelial cells. As shown in the heat map, S1 treatment enhanced secretion of multiple proinflammatory SASP factors. Figure adapted from Camell et al., Science, 2021.

RayBiotech's SARS-CoV-2 S1 Subunit Protein (RBD) (Cat# 230-30162) was used as the coating antigen in ELISA to measure plasma anti-RBD IgG titers. As shown here, Nirmatrelvir-treated mice displayed reduced humoral immune responses. Figure adapted from Fumagalli et al., EMBO Molecular Medicine, 2023.

RayBiotech’s SARS-CoV-2 RBD protein (Cat# 230-30162) was used in a microscale thermophoresis assay to show that the human SLC6A20-I529V variant reduces ACE2–RBD binding affinity, revealing how host genetics may impact viral entry. Figure adapted from Nydegger et al., Viruses, 2023.

RayBiotech’s SARS-CoV-2 S1 RBD protein (Cat# 230-30162) and Nucleocapsid protein (Cat# 230-30164) were used as ELISA capture antigens to measure vaccine-induced antibody responses. Figure shows elevated RBD- and N-specific IgG titers following ZIP1642 vaccination in mice. Figure adapted from McCafferty et al., Molecular Therapy, 2022.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

citations

  • Hybrid immunity from bivalent vaccination and prior infection enhances humoral and innate protection against Omicron XBB.1.16 and EG.5.1.1 variants in Japan

    Species:
    Human
    Sample Type:
    Other / Unspecified (monocyte-derived macrophages)
    Year:
    2026
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    42465771

  • T-cell-derived IFN-γ suppresses T follicular helper cell differentiation and antibody responses

    Species:
    Mouse
    Sample Type:
    Serum (mice immunized with RBD)
    Year:
    2025
    Journal Name:
    EMBO Journal
    PubMed ID:
    40169810

  • Antibody-independent protection against heterologous SARS-CoV-2 challenge conferred by prior infection or vaccination

    Year:
    2024
    Journal Name:
    Nature Immunology
    PubMed ID:
    38486021

  • A Paper-Based Multiplexed Serological Test to Monitor Immunity against SARS-COV-2 Using Machine Learning

    Year:
    2024
    Journal Name:
    Acs Nano
    PubMed ID:
    38888985

  • Contralateral second dose improves antibody responses to a 2-dose mRNA vaccination regimen

    Year:
    2024
    Journal Name:
    Journal of Clinical Investigation
    PubMed ID:
    38227381

  • In Vivo Iterative Adjuvant Screening Identifies an Intranasal Vaccine Formulation for Elicitation of Protective Mucosal Immune Responses Against SARS-CoV-2

    Species:
    Mouse
    Sample Type:
    Serum (vaccine antigen for immunized mice, ELISA coating antigen for mouse sera); Tissue Lysate / Homogenate (lung homogenates)
    Year:
    2025
    Journal Name:
    Pharmaceutics
    PubMed ID:
    41304760

  • SARS-CoV-2 Protein Nanoparticle Vaccines Formed In Situ From Lyophilized Lipids

    Year:
    2024
    Journal Name:
    Small
    PubMed ID:
    37849036

  • SARS-CoV-2 Spike Protein Stimulates Macropinocytosis in Murine and Human Macrophages via PKC-NADPH Oxidase Signaling

    Species:
    Mouse
    Sample Type:
    Other / Unspecified (human macrophages)
    Year:
    2024
    Journal Name:
    Antioxidants
    PubMed ID:
    38397773

  • The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction

    Year:
    2023
    Journal Name:
    Biosensors & Bioelectronics
    PubMed ID:
    36827795

  • CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation

    Species:
    Other
    Sample Type:
    Other Body Fluid (total protein or apoplastic fluid samples)
    Year:
    2025
    Journal Name:
    Plant biotechnology journal
    PubMed ID:
    39435699

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