GE100041G | Validated sgRNA targeting RFP dead mutant in GE100041C

Validated sgRNA targeting RFP dead mutant in GE100041C cells

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GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#<a href = "https://www.origene.com/catalog/vectors/crispr-vectors/ge100078-p-xcas9-guide-crispr-vector" title="GE100078" class="">GE100078</a>) or SpCas9 (Cat#<a href = "https://www.origene.com/catalog/vectors/crispr-vectors/ge100002-pcas-guide" title="GE100002" class="">GE100002</a>) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).

Specifications
Product Data
Features	Validated sgRNA to target RFP dead mutant in the RFP negative cells (GE100041C) The RFP negative cells (GE100041C) can be converted into RFP positive cells using validated sgRNA (GE100041G) and rescue donor oligo (GE100041D).
Storage	Please store at -20°C for long term storage.
Stability	The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping	Ambient

Product Manuals
Plasmid Reconstitution Protocol
FAQs
FAQ for CRISPR Vectors
SDS
SDS for GE100041G

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

Citations

Redundant and non-redundant cytokine-activated enhancers control Csn1s2b expression in the lactating mouse mammary gland
Lee, Willi, Kuhns et al
Nat Commun (2021) 12 (1), 2239 DOI: 10.1038/s41467-021-22500-w

Cited by 16

AI Summary: OriGene contributed single-guide RNAs (sgRNAs) used for CRISPR/Cas9 genome editing to generate targeted mouse models in this study.

Snippet: argeted mice were generated using C57BL/6 N mice (Charles River) by the transgenic core of the National Heart, Lung, and Blood Institute (NHLBI). Single-guide RNAs (sgRNA) were obtained from either OriGene (Rockville, MD) or Thermo Fisher Scientific (Supplementary Table 2). Target-specific sgRNAs and in vitro transcribed Cas9 mRNA were co-microinjected into the cytoplasm of fertilized eggs for

A CRISPR/Cas9 whole-genome screen identifies genes required for Aryl Hydrocarbon Receptor-dependent induction of functional CYP1A1
Sundberg, Hankinson
Toxicol. Sci. (2019)

Snippet: Page 5. non-targeting control sgRNA sequence was obtained from Origene The scrambled control sgRNA sequence was obtained from Origene (www.origene.com). Page 18. REFERENCES Androutsopoulos, Vasilis P., Aristidis M. Tsatsakis, and Demetrios A. Spandidos

p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
Awais, Spiller, White et al
Br. J. Cancer (2016)

Cited by 32

AI Summary: OriGene’s p63-turbo GFP (p63-tGFP) expression plasmid was used to transiently express p63 in uveal melanoma cells, enabling the study of p63’s role in PERP-mediated apoptosis.

Snippet: Cell transfection. Transient transfections were carried out using a p63-turbo GFP (p63-tGFP) expression plasmid (Origene, Rockville, MD, USA), which encodes full-length human protein p63 (NM_003722) transcript variant 1 tagged with tGFP. ... Cite SaveSaving

Showing 1-3 of 3 papers.

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Validated sgRNA targeting RFP dead mutant in GE100041C cells

Cat# GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

Citations

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Validated sgRNA targeting RFP dead mutant in GE100041C cells

Cat# GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

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