GE100041G | Validated sgRNA targeting RFP dead mutant in GE100041C

Validated sgRNA targeting RFP dead mutant in GE100041C cells

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GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#<a href = "https://www.origene.com/catalog/vectors/crispr-vectors/ge100078-p-xcas9-guide-crispr-vector" title="GE100078" class="">GE100078</a>) or SpCas9 (Cat#<a href = "https://www.origene.com/catalog/vectors/crispr-vectors/ge100002-pcas-guide" title="GE100002" class="">GE100002</a>) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).

Specifications
Product Data
Features	Validated sgRNA to target RFP dead mutant in the RFP negative cells (GE100041C) The RFP negative cells (GE100041C) can be converted into RFP positive cells using validated sgRNA (GE100041G) and rescue donor oligo (GE100041D).
Storage	Please store at -20°C for long term storage.
Stability	The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping	Ambient

Product Manuals
Plasmid Reconstitution Protocol
FAQs
FAQ for CRISPR Vectors
SDS
SDS for GE100041G

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Validated sgRNA targeting RFP dead mutant in GE100041C cells

Referencia GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

Citations

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Validated sgRNA targeting RFP dead mutant in GE100041C cells

Referencia GE100041G

Validated sgRNA targeting RFP dead mutant in GE100041C cells

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Citations