Atto-conjugated anti-guinea pig primary antibodies are fluorescent reagents developed for the direct detection of guinea pig antigens in immunological assays. Covalent attachment of highly photostable Atto fluorophores eliminates the need for secondary antibodies, simplifying staining protocols while reducing background and cross-reactivity.

Fluorescence Properties

Atto dyes provide exceptional brightness, high quantum yields, excellent photostability, and broad spectral coverage from green to near-infrared wavelengths. Popular variants such as Atto 488, Atto 594, Atto 633, and Atto 647N support sensitive single- and multicolor fluorescence imaging with minimal photobleaching.

Applications and Advantages

• Direct immunofluorescence: Enables rapid antigen detection without secondary antibodies.
• Broad compatibility: Suitable for immunofluorescence, immunohistochemistry, immunocytochemistry, Western blotting, and selected flow cytometry applications.
• Reliable performance: Controlled conjugation preserves antibody specificity while delivering stable fluorescence, making these reagents ideal for multiplex imaging and same-species antibody colocalization studies.