Human tetramers - Class II - PE
MHC tetramers are complexes of four MHC molecules, associated with a specific peptide and bound to a fluorochrome. Class II tetramers bind to a distinct population of CD4+ T cells.
MHC class II tetramer staining is more technically challenging than class I tetramer staining.
- Antigen-specific CD4+ T cells are more rare than antigen-specific CD8+ T cells
- Affinity between the T cell receptor and MHC/peptide complex is generally lower
- Acquisition of 100,000-200,000 CD4+ T cells is typically required
- Exclusion gating essential
- T cell expansion and/or pre-enrichment may be required
MHC tetramers are labeled with fluorescent molecules including phycoerythrin (PE), allophycocyanin (APC), or Brilliant Violet 421 (BV421) and thus allow detection of antigen-specific T cells by flow cytometry or fluorescence microscopy.
Risultati della ricerca : 34 prodotto(i) trovato(i)
Limita la ricerca :
RUOCE / IVD
- PE 34
- human 34
- Protein/peptide 34
- human 34
Cat#
Descrizione
Cond.
Priced
‹
›