BCS1L Polyclonal Antibody
Anti-BCS1L Antibody
Anti-BCS1L Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9Y276 |
| Gene Id | 617 |
| Gene Symbols | BCS1L |
| Protein Name / Synonyms | BCS1L, BCS, BCS1, BJS, FLNMS, GRACILE, Hs.6719, MC3DN1, PTD, h-BCS, h-BCS1, mitochondrial chaperone BCS1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
47kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 33-240 of human BCS1L (NP_004319.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BCS1L antibody (144-07647) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using BCS1L antibody (144-07647) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BCS1L antibody (144-07647) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BCS1L antibody (144-07647) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.