Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Dengue virus NS2B protein antibody

Dengue virus NS2B protein antibody

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Identifiez-vous

Référence GTX124246-25ul

Conditionnement : 25ul

Marque : GeneTex

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Téléphone : +1 850 650 7790

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dengue virus 2
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX124246 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Dengue virus 2 infect BHK-21

Product Note

This antibody was raised against Dengue virus 2 NS2B protein, which may cross react with NS2B protein of Dengue virus 3 but does not recognize with NS2B protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 NS2B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 2B , Dengue Virus NS2B , DENV Non-structural protein 2B , DENV NS2B

DATA IMAGES

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extract (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

The data was published in the journal Virulence in 2018. PMID: 29338543

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

The data was published in the journal Virulence in 2018. PMID: 29338543

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

The data was published in the journal Cell Rep in 2019.PMID: 31067454

Application Reference

D Kiemel et al. Nat Commun 2024; 15 (1) : 6080 Pan-serotype dengue virus inhibitor JNJ-A07 targets NS4A-2K-NS4B interaction with NS2B/NS3 and blocks replication organelle formation
Lai JH et al. Sci Rep 2023; 13 (1) : 20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12 : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6) : 102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : WB
Submit a Reference

REVIEW

Dengue virus NS2B protein antibody Cat. No. GTX124246

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Dengue virus 2 infected Huh-7 cells
Amount used : 10μg
Blocking : 5% skin milk, 25°C, 3Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane1:Huh-7 cells, Lane2:Dengue virus 2 infected Huh-7 cells
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Application Reference
D Kiemel et al. Nat Commun 2024; 15 (1):6080 Pan-serotype dengue virus inhibitor JNJ-A07 targets NS4A-2K-NS4B interaction with NS2B/NS3 and blocks replication organelle formation
Lai JH et al. Sci Rep 2023; 13 (1):20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12:926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6):102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Su YC et al. FASEB J 2020; 34 (6):7283-7294 MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Li H et al. Neuron 2019; (Epub) Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.
Application : WB
Chen WC et al. Virulence 2018; 9 (1):588-603 Lucidone suppresses dengue viral replication through the induction of heme oxygenase-1.
Lin CK et al. J Infect Dis 2018; (Epub) Prostasin impairs epithelial growth factor receptor activation to suppress dengue virus propagation.
Application : WB
Hélène Boigard et al. Vaccine 2018; 36 (50):7728-7736 Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : ICC/IF
Saisawang C et al. PLoS One 2018; 13 (2):e0193133 Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Liu H et al. J Virol 2017; 91 (9) Endoplasmic Reticulum Protein SCAP Inhibits Dengue Virus NS2B3 Protease by Suppressing Its K27-Linked Polyubiquitylation.
Lee JC et al. European Journal of Medicinal Chemistry 2017; 141:282-292 Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents.
Application : WB
Yu JS et al. Sci Rep 2017; 7:45171 schisandrin a inhibits dengue viral replication via upregulating antiviral * responses through stat signaling pathway.
Application : WB
Lin CK et al. Sci Rep 2017; 7:44701 Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents.
Application : WB
Yu JS et al. Antiviral Res 2016; 137:49-57 celastrol inhibits dengue virus replication via up-regulating type i * and downstream *-stimulated responses.
Application : WB
Tseng CK et al. Sci Rep 2016; 6:32176 Human heme oxygenase 1 is a potential host cell factor against dengue virus replication.
Application : WB
Lin CK et al. Planta Med 2016; Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication.
Application : WB
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Lee JC et al. Antiviral Res 2015; 116:43109 Characterization of the activity of 2'-C-methylcytidine against dengue virus replication.
Application : WB
Tseng CH et al. Eur J Med Chem 2014; 79C:66-76 Synthesis, antiproliferative and anti-dengue virus evaluations of 2-aroyl-3-arylquinoline derivatives.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

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