Fish Cortisol ELISA Kit

Référence CSB-E08487f-96T

Conditionnement : 96T

Marque : Cusabio


CSB-E08487f
Size 96T,5×96T,10×96T

Product Details

Abbreviation
COR
Species
Fish
Sample Types
serum, plasma, tissue homogenates.
Detection Range
3.12 ng/ml-200 ng/ml.
Sensitivity
1.56 ng/ml.
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
 
Linearity
 
Recovery
 
Typical Data
 
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months

Citations

  • SM PARUI, J Mitra, S Banerjee, A Gupta,Indian Journal of Experimental Biology (IJEB),2026
  • GR Sallam, M Hamdy, MF El Basuini, SY El-Zaeem,Scientific Reports,2025
  • C Piyavorasakul, S Triwutanon,Journal of Fisheries and Environment,2025
  • YU Kim, YJ Seo, WY Lee, SY Oh,Ocean Science Journal,2026
  • R Sharker, J Naeem, P Roy, P Datta, A Alam,Journal of Agriculture and Food Research,2025
  • MR Sharker, MJ Naeem, P Roy, P Datta, MA Alam,Journal of Agriculture and Food Research,/
  • CHP Satiti, AR Faqih, M Mahmudi,The Journal of Experimental Life Science,2025
  • S Lee, D Aulia, T Kilic, H Kim, H Jeon, S Yoon,SSRN,/
  • S Cheyadmi, H Chadli, ME Maadoudi, S Sedki,Fish Physiology and Biochemistry,2025
  • MC Hildebrand, D Runge, B Bassmann, HW Palm,Fishes,2025

Customer Reviews and Q&A

Average Rating:
5.0 - 1 reviews

Sample type: Serum

Sample species: Fish

Sample dilution: No dilution

Review: I used the kit CSB-E08487f to test tilapia serum under temperature stress. The serum was not diluted. The test results showed that the standard curve was normal, and the cortisol content in the sample was in the range of 50-200ng/mL. The experimental methods and steps of the product are deta...

By Anonymous

 Q&A
Q:

1) Does the kit come with PBS -- is that what the included was solution is? Would Tris-HCL work as a wash solution? It is what my lab wrote they used in the pilot study but I do not have any context for why they chose it over PBS.
2) For getting tissue homogenates, do you happen to have any recommendations for grinding the tissue?

A:

This kit (CSB-E08487f) is ready to use and provides all required reagents. No extra solution is required. Tris-HCl you mentioned may be not suitable for this kit. Please use the matched reagents of this kit, including the wash buffer.
For the processing of tissue samples, you need to prepare 1×PBS for homogenization treatment, use a conv...

Q:

Does this kit measure total cortisol levels, or only free/unbound cortisol? I will be using zebrafish tissue homogenates but I am not seeing any information in the protocol about whether it is total or only free cortisol.

A:

This is a competitive kit, and it detects endogenous total cortisol. For zebrafish tissue samples, it is recommended to arrange a gradient pre-experiment.

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