Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system. FFPE tissue sections require antigen retriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling at RT for 15-20 minutes. Immunofluorescence. Recommended Positive Control: Human brain from Alzheimer's disease.
Reactivity
Bovine, Equine, Human, Mouse, Rat
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Purified Human Ubiquitin.
Specificity
This antibody stains senile plaques and neurons. Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.
Buffer
PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration
lot specific
Purification
Protein A Chromatography
Conjugation
Unconjugated
Storage
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin; it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer’s disease, lewy bodies in Parkinson’s disease, pick bodies in Pick’s disease, inclusions in motor neuron disease, Mallory’s Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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