Epilepsy, Progressive Myoclonus Type 2A (Lafora Disease, EPM2A, Laforin, LD, LDE, EPM2, MELF, LAFPTPase, FLJ11207, KIAA0766)

Laforin is encoded by the EMP2A gene and plays a critical role in the survival of neurons in the brain. Laforin is also expressed in many other cells throughout the body and has mutliple functions. To carry out these functions, laforin interacts with several other proteins, including malin (which is produced from the NHLRC1 gene). These proteins are part of complex networks that transmit chemical signals and break down unneeded or abnormal proteins. Mutations in the EPM2A gene lead to the major features of Lafora progressive myoclonus epilepsy. Additionally, laforin may act as a tumor suppressor protein.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:6000
Western Blot: 0.1-0.3ug/ml. ~38kD band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (MW 37.2kD).
Immunohistochemistry(Paraffin): 2.5-3.8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.