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> 1-Monopalmitin [542-44-9]

1-Monopalmitin [542-44-9]

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Cat# HY-W009141-250mg

Size : 250mg

Brand : MedChemExpress


Description

1-Monopalmitin (Glyceryl palmitate) is an activator of the PI3K/Akt pathway and an inhibitor of P-glycoprotein (P-gp). 1-Monopalmitin can induce G2/M arrest and caspase-dependent apoptosis in cancer cells, while inhibiting IAPs protein expression. 1-Monopalmitin can increase drug accumulation by inhibiting P-gp activity in intestinal Caco-2 cells. 1-Monopalmitin has the ability to induce protective autophagy and apoptosis of lung cancer cells (IC50=50-58 μg/mL), with low toxicity to normal cells[1][2].

In Vitro

1-Monopalmitin (0-100 μg/mL; 48 h) inhibits cell proliferation in lung cancer cells (A549, SPC-A1, H460, H1299) in a dose-dependent manner with low toxicity to normal bronchial epithelial cells HBE[1].
1-Monopalmitin (50 μg/mL; 20 h) induces G2/M arrest in A549 and SPC-A1 cells, upregulates p21 protein and downregulates Cyclin D1[1].
1-Monopalmitin (12.5-50 μg/mL; 48 h) promotes apoptosis of A549 and SPC-A1 cells, activates Caspase-3 and PARP cleavage, and inhibits the expression of inhibitor of apoptosis proteins (IAPs)[1].
1-Monopalmitin (50 μg/mL; 8 h) induces protective autophagy in A549 and SPC-A1 cells, as evidenced by LC3-II accumulation and p62 degradation[1].
1-Monopalmitin (0.1-300 μM; 2 h) increases Rhodamine-123 (HY-D0816) and Daunorubicin (HY-13062A) accumulation in a dose-dependent manner in intestinal Caco-2 cells, inhibits P-glycoprotein (P-gp) activity with non-cytotoxictiy[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1-Monopalmitin Related Antibodies

Cell Viability Assay[1]

Cell Line: A549, SPC-A1 (lung cancer cells); H460, H1299 (lung cancer cells); HBE (normal human bronchial epithelial cells)
Concentration: 0, 12.5, 25, 50, 75, 100, 150, 200 μg/mL
Incubation Time: 48 h
Result: Significantly reduced viability of A549 (IC50=50.12 μg/mL) and SPC-A1 (IC50=58.30 μg/mL) cells in a dose-dependent manner.
Showed higher IC50 values against H460 and H1299 cells of 176.75 and 165.53 μg/mL, respectively; while exhibited IC50 of 161.34 μg/mL against normal HBE cells, indicating minimal toxicity to normal cells.
Molecular Weight

330.51

Formula

C19H38O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(CO)COC(CCCCCCCCCCCCCCC)=O
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (151.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (151.28 mM; warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0256 mL 15.1281 mL 30.2563 mL
5 mM 0.6051 mL 3.0256 mL 6.0513 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Purity & Documentation
References