Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CtIP (Thr847) Antibody

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Cat# p1012-847

Size : 100uL

Brand : Phosphosolutions

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Anti-CtIP (Thr847) Antibody

Our Anti-CtIP (Thr847) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human CtIP (Thr847) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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Species Reactivity

Human

Applications

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1012-847

Ships: 1-2 business days

Product Details

Target

CtIP (Thr847)

Scientific Background

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

RBBP8

Molecular Weight

100 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr847 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Human

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Chicken, Finch, Goat, Guinea Pig, Non-Human Primate, Mouse, Sheep

Antibody Registry ID

AB_2651149

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Thr847. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr847

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

COM1 antibody, COM1_HUMAN antibody, CtBP interacting protein antibody, CtBP-interacting protein antibody, CtIP antibody, DNA endonuclease RBBP8 antibody, JWDS antibody, RB binding protein 8 endonuclease antibody, RBBP-8 antibody, RBBP8 antibody, Retinoblastoma-binding protein 8 antibody, Retinoblastoma-interacting protein and myosin-like antibody, Rim antibody, SAE2 antibody, SCKL2 antibody, Sporulation in the absence of SPO11 protein 2 homolog antibody

UniProt ID

Q99708

NCBI Gene ID

5932

Shipping

Blue Ice

Western Blot Protocol: Download

Anti-CtIP (Thr847) Antibody

Cat# p1012-847

Brand : Phosphosolutions

Anti-CtIP (Thr847) Antibody

SKU: p1012-847

Product Details

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