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> Anti-Histone H3 Antibody

Anti-Histone H3 Antibody

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Cat# CPA3483-30ul

Size : 30ul

Brand : Cohesion Biosciences


  • Western blot analysis of Histone H3 expression in HeLa (A), A431 (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)
  • Immunohistochemical analysis of Histone H3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Histone H3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product Name:Anti-Histone H3 Antibody
Cat No:CPA3483
Source:Rabbit
Reactivity:H, M, R, B, C, P, Z
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to Histone H3
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of Histone H3 protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene Symbol：H3F3A
Alternative Names：H3.3A; H3F3; H3.3B; Histone H3.3
Entrez Gene (Human)： 3020; 3021;
Entrez Gene (Mouse)： 15078; 15081;
Entrez Gene (Rat)： 100361558; 117056;
SwissProt (Human)： P84243;
SwissProt (Mouse)： P84244;
SwissProt (Rat)： P84245;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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