Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Integrin β1 (Extracellular region) Antibody

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Cat# IM0041

Size : 100uL

Brand : Phosphosolutions

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Anti-Integrin β1 (Extracellular region) Antibody (M004)

Our Integrin β1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Integrin β1 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and LNCaP (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0041) at 1:1000 dilution.

Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M004). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-integrin β1 (IM0041) for ELISA capture of human recombinant integrin β1 extracellular region. Capture was detected by mouse monoclonal anti-integrin β1 (IM0411) followed by appropriate secondary antibody conjugated to HRP.

Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and LNCaP (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0041) at 1:1000 dilution.

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Species Reactivity

Human

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: IM0041

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Product Details

Target

Integrin β1 (Extracellular region)

Scientific Background

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M004

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

ITGB1

Molecular Weight

120-150

Immunogen

Clone M004 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the A431 epidermoid carcinoma cell line.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M004 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, A549, MDA-MB-231, and LNCaP cell lysates. The antibody also detects native recombinant human Integrin α1/β1, α2/β1, and α3/β1 complexes, but does not detect recombinant human Integrin α6/β4 or αV/β3 complexes. The antibody does not detect the denatured form of Integrin β1. Clone M004 can be used in western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well for detecting live, unfixed cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, TGB1, FNRB, MDF2, MSK12, ITGB1

UniProt ID

P05556

Shipping

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Anti-Integrin β1 (Extracellular region) Antibody

Cat# IM0041

Brand : Phosphosolutions

Anti-Integrin β1 (Extracellular region) Antibody (M004)

SKU: IM0041

Product Details

Bulk Order Anti-Integrin β1 (Extracellular region) Antibody