Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-Lamin A/C Antibody

Anti-Lamin A/C Antibody

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Cat# CQA8443-30ul

Size : 30ul

Brand : Cohesion Biosciences


  • Western blot analysis of Lamin A/C expression in MCF7 (A), A549 (B), Hela (C) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 63,74 kD)
  • Immunofluorescent analysis of Lamin A/C staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product Name:Anti-Lamin A/C Antibody
Cat No:CQA8443
Source:Mouse
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Mouse monoclonal antibody to Lamin A/C
Immunogen：Purified recombinant human LMNA protein fragments expressed in E.coli.
Purification：The antibody was purified by immunogen affinity chromatography.
Clonality：Monoclonal
Form：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
Gene Symbol：LMNA
Alternative Names：LMN1; Prelamin-A/C
Entrez Gene (Human)： 4000;
Entrez Gene (Mouse)： 16905;
Entrez Gene (Rat)： 60374;
SwissProt (Human)： P02545;
SwissProt (Mouse)： P48678;
SwissProt (Rat)： P48679;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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