Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-LIMS1 Antibody (3E803)

Anti-LIMS1 Antibody (3E803)

Print Print
Please log in

Cat# NB-64-118647-100uL

Size : 100uL

Brand : Neo Biotech


Remove All

Anti-PINCH Monoclonal Antibody (2L620)

(Synonyms: PINCH-1, PINCH1, PINCH, LIMS1) Copy Product Info

Synonyms: PINCH-1, PINCH1, PINCH, LIMS1

Catalog No. TMAC-03301 Copy Product Info
Anti-PINCH Monoclonal Antibody (2L620) is a Mouse antibody targeting PINCH. Anti-PINCH Monoclonal Antibody (2L620) can be used in WB,IF,FCM.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-PINCH Monoclonal Antibody (2L620) is a Mouse antibody targeting PINCH. Anti-PINCH Monoclonal Antibody (2L620) can be used in WB,IF,FCM.
SynonymsPINCH-1, PINCH1, PINCH, LIMS1
Ig Type
IgG1
Clone
2L620
Reactivity
Human
Verified Activity
1. Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Jurkat, Lane 3: Hela.
2. ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
Anti-PINCH Monoclonal Antibody (2L620),Anti-PINCH Monoclonal Antibody (2L620),Anti-PINCH Monoclonal Antibody (2L620)
Application
WB
IF
FCM
Recommended Dose
WB: 1/500-1/2000; IF: 1/200-1/1000; FCM: 1/200-1/400
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationPurified by protein A or protein G
AppearanceLiquid
FormulationAscitic fluid containing 0.03% sodium azide.
Concentration1 mg/mL
Research BackgroundThe protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Purified recombinant fragment of human PINCH expressed in E. Coli.
Antigen Species
Human
Gene Name
LIMS1
Gene ID
3987
Protein Name
LIM and senescent cell antigen-like-containing domain protein 1
Uniprot ID
P48059
Chemical Properties
Molecular WeightTheoretical: 37 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Dilution Calculator
  • Reconstitution Calculator
Related Tags: Anti-PINCH Monoclonal Antibody (2L620) molecular weight