Catalase (CAT) Activity Assay Kit
Cat# MBS9718961-96
Size : 96Tests
Brand : MyBiosource
Catalase (CAT) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9718961PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Principle of the Assay: Catalase (CAT, EC 1.11.1.6) is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) into water and oxygen. It is widely found in aerobic cells containing cytochrome systems. Hydrogen peroxide is… More details
Product Overview
Online Datasheet in PDFProduct Name
Catalase (CAT), Assay Kit
Full Product Name
Catalase (CAT) Activity Assay Kit
Product Gene Name
CAT assay kit
Samples
Serum, Plasma, Animal and Plant Tissues, Cells
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Application Notes for CAT assay kit
Catalase Activity Assay Kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.
Other Notes
Small volumes of CAT assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CAT purchase
MBS9718961 is an assay kit targeting CAT. Also known as Catalase (CAT) Activity Assay Kit.
Additional Details
NCBI/Uniprot data below describe general gene information for CAT. It may not necessarily be applicable to this product.
NCBI Official Full Name
catalase
NCBI Official Synonym Full Names
catalase
NCBI Official Symbol
CAT
UniProt Protein Name
Catalase
UniProt Gene Name
CAT
UniProt Entry Name
CATA_HUMAN
NCBI Protein Information
catalase
NCBI Summary for CAT
This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]
Show more UniProt Comments for CAT
Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.
Protein type: Amino Acid Metabolism - tryptophan; Endoplasmic reticulum; EC 1.11.1.6; Apoptosis; Energy Metabolism - methane; Mitochondrial; Hydrolase; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: peroxisomal membrane; Golgi apparatus; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol
Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding
Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; ureteric bud development; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging
Disease: Acatalasemia
Show more Protein type: Amino Acid Metabolism - tryptophan; Endoplasmic reticulum; EC 1.11.1.6; Apoptosis; Energy Metabolism - methane; Mitochondrial; Hydrolase; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: peroxisomal membrane; Golgi apparatus; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol
Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding
Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; ureteric bud development; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging
Disease: Acatalasemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with CAT assay kit
| Reference Product | PubMed Publications |
|---|---|
| PTPN11 assay kit | >4 publications with CAT and PTPN11 |
| ABL2 assay kit | >1 publications with CAT and ABL2 |
Pathways associated with CAT assay kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies | Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram |
| Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies | Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram |
| DNA Damage Response (only ATM Dependent) Pathway antibodies | DNA Damage Response (only ATM Dependent) Pathway Diagram |
| Folate Metabolism Pathway antibodies | Folate Metabolism Pathway Diagram |
| FoxO Family Signaling Pathway antibodies | FoxO Family Signaling Pathway Diagram |
| Glyoxylate And Dicarboxylate Metabolism Pathway antibodies | Glyoxylate And Dicarboxylate Metabolism Pathway Diagram |
| Glyoxylate And Dicarboxylate Metabolism Pathway antibodies | Glyoxylate And Dicarboxylate Metabolism Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Nucleotides Pathway antibodies | Metabolism Of Nucleotides Pathway Diagram |
| Oxidative Stress Pathway antibodies | Oxidative Stress Pathway Diagram |
Diseases associated with CAT assay kit
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >6454 publications with CAT and Neoplasms |
| Kidney Diseases Antibodies | >1617 publications with CAT and Kidney Diseases |
| Asthma Antibodies | >1202 publications with CAT and Asthma |
| Necrosis Antibodies | >1171 publications with CAT and Necrosis |
| Diabetes Mellitus Antibodies | >1001 publications with CAT and Diabetes Mellitus |
| Hypertension Antibodies | >733 publications with CAT and Hypertension |
| Edema Antibodies | >665 publications with CAT and Edema |
| Seizures Antibodies | >646 publications with CAT and Seizures |
| Brain Ischemia Antibodies | >532 publications with CAT and Brain Ischemia |
| Disease Progression Antibodies | >469 publications with CAT and Disease Progression |
Organs/Tissues associated with CAT assay kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >22679 publications with CAT and Brain |
| Blood Antibodies | >15407 publications with CAT and Blood |
| Nerve Antibodies | >10370 publications with CAT and Nerve |
| Muscle Antibodies | >9441 publications with CAT and Muscle |
| Eye Antibodies | >5739 publications with CAT and Eye |
| Vascular Antibodies | >4731 publications with CAT and Vascular |
| Heart Antibodies | >4571 publications with CAT and Heart |
| Liver Antibodies | >4318 publications with CAT and Liver |
| Skin Antibodies | >4068 publications with CAT and Skin |
| Kidney Antibodies | >2989 publications with CAT and Kidney |