CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

CD 81, a 26kD cell surface antigen also known as TAPA-1 and a member of the tetraspanin family. CD81 is widely expressed on human leukocytes and appears to be involved in a variety of cellular leukocytes and appears to be involved in a variety of cellular processes, including adhesion, activation, proliferation and differentiation. The antibody is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Paraffin
Flow Cytometry: 1:5-1:10. Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by researcher.

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
F(ab')2 rabbit anti-mouse IgG labeled with RPE

Hybridoma:
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice

Source: Tissue culture supernatant

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.