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> Collagen Hybridizing Peptide Control, 5-FAM Conjugate

Collagen Hybridizing Peptide Control, 5-FAM Conjugate

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Cat# FLU60CTL

Size : 60µg

Brand : 3Helix


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Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]

F-CHP is labeled with fluorescein for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,7]

Applications: immunofluorescence,[3] cell imaging,[4] SDS-PAGE (in-gel western)[7]

Specification

Synonyms F-CHP, collagen mimetic peptide (CMP)
Molecular weight 2952.01 g/mol
Purity 85% by HPLC
Conjugate Single fluorescein tag per peptide
Excitation 494 nm
Emission 512 nm
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water
Shipping Ambient temperature

Features

  • rmative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage

 

For research use only. Not intended or approved for diagnostic or therapeutic use.

 

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Citations (119)
Citations (119)
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Collagen Hybridizing Peptide, 5FAM Conjugate (F-CHP), Product Vial

Collagen Hybridizing Peptide, 5-FAM Conjugate (F-CHP)

Collagen Hybridizing Peptide, 5FAM Conjugate (F-CHP), Product Vial
Collagen Hybridizing Peptide, 5-FAM Conjugate (F-CHP)

Collagen Hybridizing Peptide, 5-FAM Conjugate (F-CHP)

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