Western Blot (Tissue lysate)
CYP17A1 polyclonal antibody (Cat # PAB7014) (0.3 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CYP17A1 polyclonal antibody (Cat # PAB7014) (3.8 ug/mL) staining of paraffin embedded human adrenal gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of CYP17A1.
Immunogen
A synthetic peptide corresponding to internal region of human CYP17A1.
Sequence
C-LFKDGDQKLEK
Host
Goat
Theoretical MW (kDa)
57.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Tissue lysate)
CYP17A1 polyclonal antibody (Cat # PAB7014) (0.3 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CYP17A1 polyclonal antibody (Cat # PAB7014) (3.8 ug/mL) staining of paraffin embedded human adrenal gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
- Gene Info — CYP17A1
Entrez GeneID
1586Protein Accession#
NP_000093.1Gene Name
CYP17A1
Gene Alias
CPT7, CYP17, P450C17, S17AH
Gene Description
cytochrome P450, family 17, subfamily A, polypeptide 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq
Other Designations
OTTHUMP00000020382|cytochrome P450, family 17|cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia|cytochrome p450 XVIIA1|steroid 17-alpha-hydroxylase/17,20 lyase|steroid 17-alpha-monooxygenase
- Interactomes
- Pathways
- Diseases
- Publication Reference
- CYP17 polymorphism as a risk factor of tamoxifen-induced hepatic steatosis in breast cancer patients.
Ohnishi T, Ogawa Y, Saibara T, Nishioka A, Kariya S, Fukumoto M, Onishi S, Yoshida S.
Oncology Reports 2005 Mar; 13(3):485.
- CYP17 polymorphism as a risk factor of tamoxifen-induced hepatic steatosis in breast cancer patients.