Reagents and instruments for immunology, cell biology and molecular biology.
 
> Cytokeratin, Multi (Multiple) (APC)

Cytokeratin, Multi (Multiple) (APC)

Print Print
Please log in

Cat# 172084-AF-APC-100ul

Size : 100ul

Brand : US Biological


172084-AF-APC Cytokeratin, Multi (Multiple) (APC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P19013 (CK4);|P13647 (CK5);|P02538 (CK6);|P05787 (CK8); |P13645 (CK10);|P13646 (CK13);|P05783 (CK18)
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IF IHC WB
Crossreactivity
Bo Fr Gt Gp Hu Mo Po Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1.0ug/ml
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
A431 cells, Skin, Colon carcinoma

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: SPM583|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: Keratin-enriched preparation from cultured human A431. Cellular Localization: Cytoplasmic|Specificity: Recognizes Cytokeratin, Multi. Species Crossreactivity: Human, bovine, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset and Porcine. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Keratin-enriched preparation from cultured human A431. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes Cytokeratin, Multi. Species Crossreactivity: Human, bovine, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset and Porcine.
References
1. Bartek J et. al. J Pathol, 1991, 164(3):215-24. 2. Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79. 3. Bartkova J; et al. Neoplasma, 1991, 38:439-46. 4. Kasper M. Histochemistry, 1991, 95(6):613-20.