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> Dihydrocytochalasin B [39156-67-7]

Dihydrocytochalasin B [39156-67-7]

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Cat# HY-N6701-500ug

Size : 500ug

Brand : MedChemExpress


Description

Dihydrocytochalasin B is an Actin disruptor. Dihydrocytochalasin B disrupts actin microfilament bundles, inhibits actin polymerization, and alters intracellular actin cytoskeletal structures. Dihydrocytochalasin B blocks the initiation of DNA synthesis. Dihydrocytochalasin B inhibits Calcium transport. Dihydrocytochalasin B inhibits cytokinesis and alters cell morphology. Dihydrocytochalasin B can be used in studies related to rickets[1][2][3].

In Vitro

Dihydrocytochalasin B (2-150×10-7 M; 24 h) reversibly inhibits serum-induced initiation of DNA synthesis in serum-starved Swiss/3T3 mouse fibroblasts[1].
Dihydrocytochalasin B (1-7×10-7 M) does not inhibit 3H-2-deoxyglucose uptake by proliferating Swiss/3T3 mouse fibroblasts[1].
Dihydrocytochalasin B (2-10×10-7 M; 24 h) inhibits serum-induced initiation of DNA synthesis in serum-starved Swiss/3T3 mouse fibroblasts, and this effect is fully reversible[1].
Dihydrocytochalasin B (5-25 μg/mL; 30-60 min) inhibits active calcium transport in everted ileal sacs of young rabbits in a dose-dependent manner; after 60 min of incubation, a significant increase in 45Ca accumulation in mucosal scrapings is observed at the dose of 25 μg/mL[2].
Dihydrocytochalasin B (4-10 μM; 1 h) induces dose-dependent morphological changes identical to those induced by cytochalasin B (including cytoplasmic blebbing, nuclear extrusion, dendritization, and cell rounding) in BALB/c 3T3 cells, but it has slightly lower potency and exhibits an additive effect with cytochalasin B[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dihydrocytochalasin B Related Antibodies

Immunofluorescence[1]

Cell Line: serum-arrested Swiss/3T3 mouse fibroblasts
Concentration: 1×10-7 M, 2×10-7 M, 5×10-7 M, 7.8×10-7 M, 10×10-7 M
Incubation Time: 24 h
Result: Caused concentration-dependent disruption of actin microfilament bundles, loss of cell-substratum attachment sites, and progressive cell rounding.
Detected no changes in actin structure or cell shape at 1×10-7 M.
Made microfilament bundles fewer, thinner, and shorter, with cells assuming a rectangular shape at 2×10-7 M.
Left only peripheral microfilament bundles remaining, with cells adopting a spindle shape at 5×10-7 M.
Resulted in few to no actin microfilament bundles visible, with cells becoming rounded with blebby surfaces at 7.8 and 10×10-7 M.
Increased the percentage of cells with disorganized actin structure with concentration: 11% at 1×10-7 M, 30% at 2×10-7 M, 43% at 5×10-7 M, 60% at 7.8×10-7 M, and 89% at 10×10-7 M.
Showed correlation between actin disorganization and inhibition of DNA synthesis.
Molecular Weight

481.62

Formula

C29H39NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@]2(O3)[C@]([C@@H](C([C@@H](O)[C@]2(15)/C=C/C[C@@H](CCC[C@@H](O)CCC3=O)C)=C)C)(15)[C@H](CC4=CC=CC=C4)N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : 30 mg/mL (62.29 mM; Need ultrasonic and warming)

DMSO : ≥ 20 mg/mL (41.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0763 mL 10.3816 mL 20.7633 mL
5 mM 0.4153 mL 2.0763 mL 4.1527 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

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