EDDM3B (NM_022360) Human Tagged ORF Clone
CAT#: RC213305
EDDM3B (Myc-DDK-tagged)-Human epididymal protein 3B (EDDM3B)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EDDM3B |
Synonyms | EP3B; FAM12B; HE3-BETA; HE3B; RAM2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data | >RC213305 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCGTCTGTAAAGATCTGGGGCACACTCTTGGCCCTACTTTGCATCCTATGCACACTGCTTGTAC AGAGCAAAGAAGTTTCTTGGAGAGAATTCATGAAACAGCACTACTTAAGTCCAAGTCGAGAATTCAGAGA GTACAAATGTGATGTCCTCATGAGAGAAAATGAAGCTCTGAAAGACAAGAGCTCTCACATGTTTATCTAT ATCTCATGGTACAAAATCGAGCATATATGCACTAGTGACAACTGGATGGATCGCTTCCGAAATGCATATG TATGGGTCCAGAATCCTCTCAAAGTACTCAAGTGTCACCAGGAGAATTCCAAAAATAGCTACACAGAGAG CAGGAGCTTCAACTACATTGAATTCCATTGTAGCATGGACGGGTATGTTGATAGCATAGAAGACCTAAAG ATGGTAGAACCTATCGGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213305 protein sequence Red=Cloning site Green=Tags(s) MASSVKIWGTLLALLCILCTLLVQSKEVSWREFMKQHYLSPSREFREYKCDVLMRENEALKDKSSHMFIY ISWYKIEHICTSDNWMDRFRNAYVWVQNPLKVLKCHQENSKNSYTESRSFNYIEFHCSMDGYVDSIEDLK MVEPIGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022360 |
ORF Size | 441 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022360.5 |
RefSeq Size | 897 bp |
RefSeq ORF | 444 bp |
Locus ID | 64184 |
UniProt ID | P56851 |
Cytogenetics | 14q11.2 |
Protein Families | Secreted Protein, Transmembrane |
MW | 17.6 kDa |
Gene Summary | Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
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SDS |
Resources
Other Versions
SKU | Description | Size | |
---|---|---|---|
RC213305L3 | Lenti ORF clone of Human epididymal protein 3B (EDDM3B), Myc-DDK-tagged | 450,00 EUR | |
RC213305L4 | Lenti ORF clone of Human epididymal protein 3B (EDDM3B), mGFP tagged | 450,00 EUR | |
RG213305 | EDDM3B (tGFP-tagged) - Human epididymal protein 3B (EDDM3B) | 350,00 EUR | |
SC305017 | EDDM3B (untagged)-Human epididymal protein 3B (EDDM3B) | 165,00 EUR |