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> Equine IFN-gamma ELISA Kit

Equine IFN-gamma ELISA Kit

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Cat# ELE-IFNg-1

Size : 1PlateKit

Brand : RayBiotech


25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Equine IFN-gamma ELISA KitEquine IFN-gamma ELISA KitEquine IFN-gamma ELISA KitEquine IFN-gamma ELISA KitEquine IFN-gamma ELISA KitEquine IFN-gamma ELISA Kit5 Plate Kit Packaging (one box)

Equine IFN-gamma ELISA Kit

Equine IFN-gamma ELISA Kit

RayBio® Equine IFN-gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesEquine (horse)
Sensitivity
0.13 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.13 ng/ml - 100 ng/ml
Specificity
This ELISA kits detects equine IFN-gamma. This antibody pair has less than 20% cross-reactivity with recombinant canine IFN-gamma is observed, less than 6% cross-reactivity with recombinant feline IFN-gamma and recombinant bovine IFN-gamma is observed, and less than 0.2% cross-reactivity with recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, recombinant porcine IFN-gamma, recombinant rhesus macaque IFN-gamma, and recombinant cotton rat IFN-gamma is observed.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P42160
Gene Id
100034181
Gene Symbols
IFNG
Protein Name / Synonyms
Interferon gamma (IFN-gamma)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCancer, Inflammation, Autophagy, Innate Immunity
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Equine IFN-gamma ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IFN-gamma (IFNG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELE-IFNg.pdf)
SDS (ELE-IFNg.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Effects of dietary zinc chloride hydroxide and zinc methionine on the immune system and blood profile of healthy adult horses and ponies

    Species:
    Pony
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    ARCHIVES OF ANIMAL NUTRITION