Reagents and instruments for immunology, cell biology and molecular biology.

Fast red TR salt hemi(zinc chloride) [89453-69-0]

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Cat# NB-64-88795-500mg

Size : 500mg

Brand : Neo Biotech

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Fast red TR salt hemi(zinc chloride)

(Synonyms: 4-Chloro-2-methylbenzenediazonium salt) Copy Product Info

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Synonyms: 4-Chloro-2-methylbenzenediazonium salt

Catalog No. T86407 Copy Product Info

Purity: 99.52%

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Fast red TR salt hemi(zinc chloride) is a red diazo dye with λmax = 284 nm, commonly used as a chromogenic substrate for alkaline phosphatase (AP) and in immunohisto-chemistry to detect specific proteins or antigens in tissue sections.

Cas No. 89453-69-0

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.52%

Appearance:Solid

Color:White to Yellow

Product Introduction

Bioactivity

Description	Fast red TR salt hemi(zinc chloride) is a red diazo dye with λmax = 284 nm, commonly used as a chromogenic substrate for alkaline phosphatase (AP) and in immunohisto-chemistry to detect specific proteins or antigens in tissue sections.
Synonyms	4-Chloro-2-methylbenzenediazonium salt

Chemical Properties

Molecular Weight	257.18
Formula	C₇H₆Cl₃N₂.1/2Zn
Cas No.	89453-69-0
Smiles	[Cl-][Zn+2]([Cl-])([Cl-])[Cl-].CC1=CC(Cl)=CC=C1[N+]#N.CC2=CC(Cl)=CC=C2[N+]#N

Storage & Solubility Information

Storage

Keep away from direct sunlight Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (972.08 mM), Sonication is recommended.

H2O: 80.00 mg/mL (311.07 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 10 mg/mL (38.88 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8883 mL	19.4416 mL	38.8833 mL	194.4164 mL
5 mM	0.7777 mL	3.8883 mL	7.7767 mL	38.8833 mL
10 mM	0.3888 mL	1.9442 mL	3.8883 mL	19.4416 mL
20 mM	0.1944 mL	0.9721 mL	1.9442 mL	9.7208 mL
50 mM	0.0778 mL	0.3888 mL	0.7777 mL	3.8883 mL
100 mM	0.0389 mL	0.1944 mL	0.3888 mL	1.9442 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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