Fast Red Violet LB [32348-81-5]

Fast Red Violet LB is a dye for staining tartrate resistant acid phosphatase (TRAP). Fast Red Violet LB can be used for alkaline phosphatase (ALP) activity staining^[1][2].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution^[2]
1.1 Prepare 0.1% Fast Red Violet LB solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Yan D, et al. Genetic background influences fluoride's effects on osteoclastogenesis. Bone. 2007 Dec;41(6):1036-44.  [Content Brief]

  [2]. Kotobuki N, et al. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs. 2004 Jan;28(1):33-9.  [Content Brief]