GRK5 (G-Protein-Coupled Receptor Kinase 5, G-Protein-coupled Receptor Kinase GRK5, GPRK5)

GRK5 is a 590aa protein belonging to the G protein-coupled receptor kinase family and consisting of AGC-kinase C-terminal, protein kinase and RGS domains. It phosphorylates the activated forms of G protein-coupled receptors, thus initiating their deactivation. It also phosphorylates the human substance P receptor and plays a role in regulating the motility of polymorphonuclear leukocytes (PMNs). It contains a membrane binding motif consisting of a group of hydrophobic amino acids that mediates its plasma membrane (PM) localization. GRK5 is expressed in heart, placenta, lung, skeletal muscle, brain, liver, pancreas and kidney.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry (paraffin): 20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.