Research Use Only

This is a laboratory product for research use only. Not for personal consumption. It will not be effective and is dangerous for personal use.

Description

RayBiotech's high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, the DNA polymerases produce PCR products with very high speed and accuracy. Also, they are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. Our high-fidelity DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. High-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.

High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), this is one of the most accurate thermostable polymerases available. The DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. RayBiotech's DNA Polymerase is supplied with standard 5X Buffer, which can be used for complex or GC-rich templates. Reactions can also be optimized using the provided DMSO.

Molecule Information